The new structures reveal how the enzymes are selective for DNA junctions, as well as the principles of the cleavage reaction. 新的结构揭示了这两种酶何以对DNA结合点有选择性,也揭示了分解反应的原理。
The cleavage and formation of the chemical bonds in the reaction pathway have been discussed by the topological analysis of electronic density. 对反应过程中若干关键点进行了电子密度拓扑分析,讨论了反应进程中键的断裂、生成和化学键的变化规律。
From the data we'd gotten, three of them were activated remarkably: acid invertase, sorbitol oxidase and sucrose synthase ( cleavage reaction). 结果表明在20μmol/L的ABA处理浓度下,酸性转化酶、山梨醇氧化酶和蔗糖合酶(分解方向)的激活效应最为明显;
After in vitro transcription, the cleavage reaction was shown to have cut off 79.3% target RNA in 1h. 经体外转录出核酶RZ1及靶RNA后,体外切割反应1小时,79.3%的靶RNA即被切开。
Results The products of trans cleavage were obtained 30 minutes after the initiation of the reaction and the reaction was further intensified 90 minutes later. 结果启动反应30min后,可以观察到剪切产物,90min后剪切反应更加明显。
Then the liquid-phase catalytic cleavage reaction of TDC was studied. 对TDC液相催化热分解反应进行研究。
The cleavage reaction was conducted by mixing ribozyme and its target mRNA in a buffer containing Mg 2+. 在含有Mg2+的反应体系中,进行核酶对靶mRNA的体外切割反应。
Seryl-histidine Dipeptide Side Chains Effects on Its DNA Cleavage Reaction 丝组二肽所含基团在其切割DNA反应中的作用金属有机配合物对DNA的断链作用
Therefore, the cleavage of the Hg-S bond and the proton transfer are the decisive steps for the photochromic reaction of this complex. 因此可以认为:硫汞键的断裂以及质子转移过程是该类材料能发生光色互变反应的关键性步骤。
Cleavage of Chemical Bonds via β-Elimination Reaction of Organometallic Compounds 通过金属有机化合物的β-消除切断化学键
The substrate caspase 3 mRNA fragment was generated from human peripheral blood mononuclear cells by RT PCR. After transcription of ribozymes and substrate by 32 P, cleavage reaction in vitro was detected. 应用RTPCR方法从人外周血单个核细胞扩增底物caspase3mRNA目的片段,体外转录物作为靶RNA,核酶与靶RNA按1∶1比例进行体外切割实验。
Addition of HD-1 could enhance the cleavage reaction and upgrading reaction, restrain the polymerization/ condensation reactions, reduce the gas production, and increase the recovery ratio of saturated and aromatic hydrocarbons favored. 供氢剂能够明显促进胶质和沥青质的裂解反应,抑制聚合反应,降低气体产量,提高理想产物饱和烃和芳香烃收率。
Actually, under the effect of trypsin, cleavage and coupling was a reversible reaction. 胰蛋白酶作用下的酶切与偶联反应,实际是一对可逆的反应,即酶切为肽键的断裂,偶联为肽键的形成。
According intermediates of phenanthrene degradation, phenanthrene was added hydroxyl at the action of the dioxygenase enzyme in aromatics, generated phthalic acid substances. And ring cleavage reaction occurred, organic carboxylic acids was generated. 根据中间产物可推测ZS1菌在降解菲时,首先通过双加氧酶的作用在芳烃上加氧,生成邻苯二甲酸类物质,然后发生环裂解反应生成大量有机羧酸类物质。
It is a type of important organic reactions that unsaturated fatty acids and their derivatives oxidative cleavage reaction, which dicarboxylates is prepared through. 不饱和脂肪酸及其衍生物氧化裂解制备二元羧酸,是一类重要的有机化学反应。
Although some methods have been developed for the cleavage of carbon-nitrogen bonds with metals, strong acids, or bases, they are not suitable for the development of highly selective organic reactions because of harsh reaction conditions. 虽然某些金属、强酸或强碱等试剂可以断裂碳-氮键,但是反应条件比较剧烈,不适合于发展高选择性的有机化学反应。
The transition-metal catalyzed C-H bond activation and C-C bond cleavage reaction is a hotspot in modern organic synthesis. 过渡金属催化的C-H键羟基化和C-C键裂解的反应是现代有机合成反应的热点研究领域,吸引着越来越多有机化学家的关注。
After cleavage from resin, only the target peptide could be absorbed by gold nanoparticles, because the unreacted amino groups is capped by the addition of acetic anhydride and pyridine afte every step of reaction coupling amino acid. 因为合成过程中,每一步使用乙酸酐和吡啶封闭未反应的氨基,所以只有目标多肽会通过半胱氨酸的巯基与纳米金作用。
At the cleavage stage positive reaction mainly distributed in cell membranes and nuclear round about. 卵裂期阳性反应主要分布于细胞膜和核的周围。
This cascade reaction proceeds with high stereoselectivity through a C-N bond cleavage along with a new C-N bond formation as well as an intramolecular aldol reaction. 这个串联反应涉及到旧的碳氮键的断裂和新碳氮键的形成以及分子内的aldol反应。
Therefore, this paper establishes a catalytic cleavage system of carboxylic esters, which has such characteristics as mild reaction conditions, high efficiency and specificity etc. So it is a new method to decompose the carboxylic esters. 因此,本论文建立了近中性条件下催化羧酸酯分解反应的催化体系,此体系具有反应温和、高效、专一等特点,是分解羧酸酯的新方法。
The results of these calculations illustrate the introduction of zinc finger protein of DNA detection agent to enhance the copper nuclease DNA cleavage specificity. Thirdly, this section consists of two organic reaction mechanism. 这些计算结果说明,DNA识别剂的锌指蛋白的引入,很好地提升含铜核酸酶的DNA切割特异性。三.本部分主要研究了两个有机反应机理。